Operator binding by lambda repressor heterodimers with one or two N-terminal arms.
نویسندگان
چکیده
منابع مشابه
One nanosecond molecular dynamics simulation of the N-terminal domain of the lambda repressor protein.
We have carried out molecular dynamics simulation of the N-terminal domain of the lambda repressor protein in a surrounding environment including explicit waters and ions. We observe two apparent dynamics substates in the nanosecond protein simulation, the transition occurring around 500 ps. The existence of these two apparent substates results from a high flexibility of the arm in each monomer...
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sites separated by 3–7 bp linkers. The repressor binds to each of these sites as a dimer. Because the operator sites are not identical in sequence, the intrinsic affinity of the repressor for each of the individual sites varies, Interactions between transcription factors bound to a lysogen. The cooperatively bound pair of repressor separate operator sites commonly play an important dimers preve...
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The recognition of operator DNA by Tet repressor was analyzed by fluorescence stopped flow measurements. The main part of the fluorescence change observed for the reaction of the repressor with operator DNA reflects a second-order binding reaction including the expected concentration dependence. Global fitting of transients measured at different concentrations reveal at least one intramolecular...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1995
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.92.16.7510